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Glioma 35(strain)
transplantable tissue strain / Rattus norvegicus / Glioma
- Animal species:
- Brown rat Rattus norvegicus.
- Age:
- newborn animals.
- Tissue:
- brain.
- Histological diagnosis:
- Glioma
- Carcinogenesis:
- Ethylnitrosourea (to newborn rats intracerebrally)
- Tumorigenicity (%):
- 80-100% in Wistar rats, brown rat, hooded rat.
- Optimal cell number:
- 20 µL of minced tissue, about 8×10⁵ cells.
- Contamination control:
- bacteria, fungi and mycoplasma not detected.
- Viral infection control:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Molecular profile:
- Ki 67 index (Me, %): Mitotic figures (Me, per high-power field):
- Karyology:
- 2n=42, range of variation in chromosome number: first clone in the cell population 41-44, second clone 81-86. modal chromosome number 81-82 and number of markers - 6 (differential staining).
- Cell line obtained:
- no
- Tumoroids obtained:
- no
- Subcutaneous growth:
- yes
- Application area:
- neuro-oncology, cell biology.
- Culture method:
- transplantation of fresh/thawed tissue orthotopically or subcutaneously (see Appendix 1).
- Culture conditions:
- in vivo tissue fresh or thawed after deep freezing (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing). Histological description:
- History:
- obtained at the A.P. Romodanov Institute of Neurosurgery, National Academy of Medical Sciences of Ukraine
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", KKKP INC RAS
- Author:
- A.P. Romodanov Institute of Neurosurgery (Zhmareva E.N.)
- Patent, certificate:
- no
- Patents, scientific publications using these samples:
- Obtained from the Research Institute of Neurosurgery (Kyiv), 1981. Atlas of chromosomes of permanent human and animal lines, S.E. Mamaeva, 2002. M. Nauchny Mir. Electron microscopic study of transplantable rat gliomas. Likhtenshtein V.E., Zhmareva E.N., Pinchuk V.G., Experimental Oncology, 1983.5.No. 3. 56-61
- Note:
- intracerebral and subcutaneous growth, i.v. (2×10⁵) tumors in the lungs
- Intended use:
- these samples are intended for laboratory research only.