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WI-26 VA4
cell line / Homo sapiens / epithelial-like
- Lot number:
- WI-26VA4-05-20
- Deposit date:
- 21.05.2020
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Cell growth properties:
- adherent
- Species identity control:
- STR profiling CSF1PO 10, 11; D10S1248 15, 16; D12S391 19, 24; D13S317 12; D16S539 9, 11; D18S51 17; D19S433 13, 16.2; D1S1656 14, 17.3; D21S11 29, 30; D22S1045 16; D2S1338 19, 25; D2S441 11; D3S1358 14, 16; D5S818 11, 12; D6S1043 11, 19; D7S820 10, 13; D8S1179 14; DYS391 10; FGA 25; GENDER XY; SE33 20, 29.2; TH01 6, 7; TPOX 10, 11; vWA 17, 20; Yindel 2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:2 – 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %
- Karyotype:
- Distribution of chromosome frequencies in 49 cells: 2n = 46 The karyotype is unstable within the triploid stemline, ranging from 67 to 71.
- Viability after cryopreservation:
- 95% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology
- Safety declaration:
- The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms