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THP-1
cell line / Homo sapiens / monocyte
- Lot number:
- THP1-12-21
- Deposit date:
- 8.12.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- THP-1 (ATCC)
- Origin:
- Homo sapiens, boy, 1 year old, acute monocytic leukemia
- Morphology:
- monocyte
- Species identity control:
- STR profiling CSF1PO : 11,13. D10S1248: 14. D12S391: 19. D13S317: 13. D16S539: 11,12. D18S51: 13,14. D19S433: 12.2,13. D1S1656: 16,17. D21S11: 30,31.2. D22S1045: 11,15. D2S1338: 17,18. D2S441: 10,11. D3S1358: 15,17. D5S818: 11,12. D6S1043: 14. D7S820: 10. D8S1179: 10,14. DYS391: 10. FGA: 24,25. GENDER: X,Y,SRY. SE33: 20,30.2. TH01: 8,9.3. TPOX: 8,11. vWA: 16. Yindel: 1
- Tumorigenicity:
- non-tumorigenic in Balb/c nude mice
- Phenotypic markers (expressed antigens):
- CD45, CD14, PD-L1, ERα, ERβ, TUBB3, EMT
- Cell growth properties:
- suspension
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 97% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology