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sp2/0-Ag14
cell line / Mus musculus / lymphoblast-like
- Lot number:
- sp2/0-Ag14-12-21
- Deposit date:
- 9.12.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor PHOTO
- Synonyms and presence in other collections:
- sp2/0-Ag14 (ATCC)
- Origin:
- Mus musculus (B cell). Mus musculus (myeloma), hybridoma
- Morphology:
- lymphoblast-like
- Tumorigenicity:
- tumorigenic in Balb/c mice
- Phenotypic markers (expressed antigens):
- H-2d
- Cell growth properties:
- suspension
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 96% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology