English
RWH4
hybridoma / Mus musculus / lymphoblast-like
- Lot number:
- RWH4
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Origin:
- mouse, Mus musculus, myeloma, hybrid of sp2/0-Ag14 and splenocytes of BALB/c mice
- Morphology:
- lymphoblast-like
- Tumorigenicity:
- tumorigenic in Balb/c mice
- Phenotypic markers (expressed antigens):
- H-2d
- Cell growth properties:
- suspension
- Culture conditions:
- medium – DMEM with 4.5 mg/L glucose, fetal bovine serum 4%, 4 mM L-glutamine, sodium pyruvate 110 µg/mL, sodium bicarbonate 3.7 g/L, gentamicin (10 µg/mL). 37 °C, 5% CO2.
- Subculture procedure:
- 2 -3 times a week depending on cell density, maintain a density of 3-9 x 100 000 cells/mL, split ratio 1:5
- Cryopreservation:
- DMEM, fetal bovine serum 40%, DMSO 10 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 84% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology