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RONC-RCP (RONC-RCP-11-23)
cell line / Homo sapiens / epithelial-like
- Deposit date:
- 23.11.2023
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Unique line
- Origin:
- Homo sapiens, male, 72 years old, clear cell renal cell carcinoma (lymph node metastasis)
- Morphology:
- epithelial-like
- Age (number of passages):
- 40 passages
- Species identity control:
- STR profiling CSF1PO: 10, 12. D10S1248: 14. D12S391: 20. D13S317: 11, 12. D16S539: 12. D18S51: 11, 17. D19S433: 14. D1S1656: 12, 15. D21S11: 30, 33.2. D22S1045: 11, 15. D2S1338: 21. D2S441: 10, 12. D3S1358: 17. D5S818: 11, 13. D6S1043: 14, 16. D7S820: 10, 12. D8S1179: 12, 14. DYS391: -. FGA: 19, 23. GENDER: X. SE33: 22.2, 29.2. TH01: 6, 9. TPOX: 8. vWA: 14, 18.Yindel: -
- Tumorigenicity:
- non-tumorigenic in Balb/c nude mice
- Immunohistochemistry:
- Her2++, EGFR++, CD63–, Topo2α+, TUBB3++, Bax–, N-cadh+, E-cadh+, MMP9–, NSE+
- Flow cytometry:
- TUBB3+, ERβ+, PD-L1+, EMT+
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 98% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology