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RONC-mel-63 (RONC-mel-63-11-23)
cell line / Homo sapiens / epithelial-like
- Deposit date:
- 23.11.2023
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Unique line
- Origin:
- Homo sapiens, male, 62 years old, skin melanoma, lymph node metastasis
- Morphology:
- epithelial-like
- Age (number of passages):
- 38 passages
- Species identity control:
- STR profiling CSF1PO: 10, 11. D10S1248: 13, 15. D12S391: 19. D13S317: 8, 10. D16S539: 9, 12. D18S51: 12, 14. 19S433: 16, 17.2. D1S1656: 15.3, 17. D21S11: 29, 30, 31. D22S1045: 14, 17. D2S1338: 20, 25. D2S441: 10. D3S1358: 14, 18. D5S818: 10, 12. D6S1043: 13, 16. D7S820: 11, 12. D8S1179: 12. DYS391: 10. FGA: 20, 25. GENDER: XY. SE33: 13, 18. TH01: 9. TPOX: 11, 12. vWA: 17. Yindel: 2
- Tumorigenicity:
- non-tumorigenic in Balb/c nude mice
- Immunohistochemistry:
- Her2–, EGFR–, CD63–, Topo2α+, TUBB3+++, Bax++, N-cadh+, E-cadh–, MMP9–, NSE–
- Flow cytometry:
- TUBB3+, ERβ+, PD-L1+
- Cell growth properties:
- suspension
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, split ratio 1:8
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 99 % (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology