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MNNG-HOS (2023-08-002)
cell line / Homo sapiens / epithelial-like
- Deposit date:
- 07.08.2023
- Number of cells per ampoule:
- 10,0×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Shelf life:
- unlimited
- Synonyms and presence in other collections:
- MNNG-HOS Cl #5, CRL-1547 (ATCC)
- Origin:
- Homo sapiens, female, 13 years old, osteosarcoma
- Morphology:
- epithelial-like
- Age (number of passages):
- more than 100.
- Species identity control:
- STR profiling CSF1PO: 12. D10S1248: 13, 16. D12S391: 20. D13S317: 12. D16S539: 10, 13. D18S51: 14. D19S433: 13. D1S1656: 13. D21S11: 31.2. D22S1045: 14, 16. D2S1338: 24, 25. D2S441: 10. D3S1358: 15. D5S818: 13. D6S1043: 18. D7S820: 11, 12. D8S1179: 11, 14. DYS391: -. FGA: 24. GENDER: X. SE33: 20, 21. TH01: 6. TPOX: 8, 11. vWA: 18. Yindel: -
- Karyotype:
- no data
- Tumorigenicity:
- tumorigenic in Balb/c nude mice
- Cell growth properties:
- adherent
- Culture conditions:
- medium - RPMI 1640 fetal bovine serum 10% L-glutamine - 2mM
- Subculture procedure:
- cell detachment using 0.05% trypsin-EDTA , split ratio 1:2 - 1:4
- Cryopreservation:
- growth medium, 10% RPMI-1640, 10.0×10⁶ cells/mL in ampoule
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing 21.08.2023)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HPV, EBV, CMV and HHV6 viruses not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology