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MG-63 (2023-07-001)
cell line / Homo sapiens / fibroblast-like
- Deposit date:
- 31.07.2023
- Number of cells per ampoule:
- 2,0×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Shelf life:
- unlimited
- Synonyms and presence in other collections:
- CRL-1427 (ATCC)
- Origin:
- Homo sapiens, male, 14 years old, osteosarcoma
- Morphology:
- fibroblast-like
- Age (number of passages):
- more than 100
- Species identity control:
- STR profiling CSF1PO: 10, 12. D10S1248: 15, 16. D12S391: 15. D13S317: 11. D16S539: 11. D18S51: 16. D19S433: 13, 14. D1S1656: 14, 16. D21S11: 30. D22S1045: 16. D2S1338: 17, 24. D2S441: 10, 11. D3S1358: 15. D5S818: 11, 12. D6S1043: 12, 17. D7S820: 10. D8S1179: 13. DYS391: 11. FGA: 21, 25. GENDER: XY. SE33: 20, 26.2. TH01: 9.3. TPOX: 8, 11. vWA: 16, 19. Yindel: 2.
- Karyotype:
- Hypotriploid human cell line. The modal chromosome number was 66 and occurred in 44% of cells. The proportion of cells with higher ploidy was 2.0%. Eighteen to 19 marker chromosomes are common to all cells. (according to ATCC)
- Tumorigenicity:
- non-tumorigenic in Balb/c nude mice
- Phenotypic markers (expressed antigens):
- TGF-beta RI +, TGF-beta) RII+ (according to ATCC)
- Cell growth properties:
- adherent
- Culture conditions:
- medium - RPMI 1640 fetal bovine serum 10% L-glutamine - 2mM
- Subculture procedure:
- cell detachment using 0.05% trypsin-EDTA , split ratio 1:2 - 1:4
- Cryopreservation:
- growth medium, 10% RPMI-1640, 2.0×10⁶ cells/mL in ampoule
- Viability after cryopreservation:
- 90% (trypan blue staining after thawing 17.08.2023)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HPV, EBV, CMV and HHV6 viruses not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology