English
mel IL
cell line / Homo sapiens / mixed-cell, polymorphic
- Lot number:
- melIL-11-21
- Deposit date:
- 01.11.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Unique line
- Origin:
- Homo sapiens, male, skin melanoma (metastasis) [Human melanoma cell lines. Monograph / Ed. by I.N. Mikhailova and M.M. Davydov. – St. Petersburg: Naukoemkie Tekhnologii, 2012. – 174 pp.]
- Morphology:
- mixed-cell, polymorphic
- Age (number of passages):
- 35 passages
- Species identity control:
- STR profiling CSF1PO: 11. D10S1248: 14,15. D12S391: 17,18. D13S317: 11. D16S539: 9. D18S51: 12. D19S433: 13,14.2. D1S1656: 16,18.3. D21S11: 29,33.2. D22S1045: 15. D2S1338: 23,25. D2S441: 11,14. D3S1358: 18. D5S818: 11. D6S1043: 17. D7S820: 11,13. D8S1179: 12,13. DYS391: -. FGA: 22,25. GENDER: X. SE33: 19. TH01: 8. TPOX: 8,11. vWA: 17,18. Yindel: -
- Karyotype:
- 56~61,XX, Y,+1,+del(1)(q21),+add(2)(q37.3), der(9.14)(q10.q10)×2,+15,der(15.22)(q10.q10)×2, add(17)(q25), +20,der(20)t(7.20)(q11.p12)×3,+22,+1~2mar, inc
- Tumorigenicity:
- tumorigenic in Balb/c nude mice
- Phenotypic markers (expressed antigens):
- CD63, HMB45, Melan A, PD-L1, ERα, ERβ, TUBB3, EMT
- Mutations:
- BRAF
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 98% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology