English
Jurkat clon E6
cell line / Homo sapiens / lymphoblast
- Lot number:
- Jurkat-11-21
- Deposit date:
- 22.11.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Jurkat, Clone E6-1 (ATCC)
- Origin:
- Homo sapiens, male, 14 years old, acute T-lymphoblastic leukemia
- Morphology:
- lymphoblast
- Species identity control:
- STR profiling CSF1PO : 10,11,12. D10S1248: 14,16. D12S391: 22,23,24. D13S317: 8,11. D16S539: 11,12. D18S51: 13,21. D19S433: 13,15.2. D1S1656: 16,16.3. D21S11: 31.2,33.2. D22S1045: 14,16. D2S1338: 19,23. D2S441: 13,14. D3S1358: 14,16,17. D5S818: 9. D6S1043: 11. D7S820: 8,10. D8S1179: 11,14,15. DYS391: 12. FGA: 20,21,22. GENDER: X,Y,SRY. SE33: 17,19. TH01: 6,9.3. TPOX: 8,10. vWA: 18,19. Yindel: 2
- Karyotype:
- 46,XY,-2,-18,del(2) (p21p23),del(18) (p11.2).
- Tumorigenicity:
- tumorigenic in Balb/c nude mice
- Phenotypic markers (expressed antigens):
- CD3, CD4, CD5, CD7, PD-L1, ERα, ERβ, TUBB3
- Cell growth properties:
- suspension
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- 2-3 times a week, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 60% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology