English
HT-1080 (2023-08-003)
cell line / Homo sapiens / epithelial-like
- Deposit date:
- 14.08.2023
- Number of cells per ampoule:
- 2,1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Shelf life:
- unlimited
- Synonyms and presence in other collections:
- CCL-121 (ATCC)
- Origin:
- Homo sapiens, male, 35 years old, fibrosarcoma
- Morphology:
- epithelial-like
- Age (number of passages):
- more than 100
- Species identity control:
- STR profiling CSF1PO: 12. D10S1248: 13, 15. D12S391: 20, 22. D13S317: 12, 14. D16S539: 9, 12. D18S51: 12, 18. D19S433: 13.2, 15. D1S1656: 16, 17.3. D21S11: 28, 30. D22S1045: 11, 16. D2S1338: 25, 26. D2S441: 10, 14. D3S1358: 16. D5S818: 11, 13. D6S1043: 12, 18. D7S820: 9, 10. D8S1179: 13, 14. DYS391: 10. FGA: 22, 25. GENDER: XY. SE33: 21.2, 30.2. TH01: 6. TPOX: 8. vWA: 14, 19. Yindel: 2
- Karyotype:
- Modal number 46. range from 44 to 48.Pseudodiploidy is often noted. About 40% of cells have rearranged karyotypes with an additional chromosome of group E and absence of a chromosome of group C, probably chromosome 11. (according to ATCC)
- Tumorigenicity:
- tumorigenic in nude mice
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL) 37 °C, 5% CO2
- Subculture procedure:
- cell detachment using 0.05% trypsin-EDTA , split ratio 1:2 - 1:4
- Cryopreservation:
- growth medium, 10% RPMI-1640, 2.1×10⁶ cells/mL in ampoule
- Viability after cryopreservation:
- 98% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HPV, EBV, CMV and HHV6 viruses not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology