English
T47-D
cell line / Homo sapiens / epithelial
- Lot number:
- T47D-12-21
- Deposit date:
- 06.12.2021
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- ATCC HTB-133. DSZM ACC 739
- Origin:
- Homo sapiens, female, 54 years old, infiltrating ductal carcinoma of the breast
- Morphology:
- epithelial
- Species identity control:
- STR profiling
- Karyotype:
- Hypotriploid line. The modal chromosome number is 65, occurring in 50%, polyploidy is 0.8%. 18 marker chromosomes are common to most cells, of which 7 are paired and 11 are single-copy. T (8q 14q), t (9q 17q), t (10q17p) are among the 7 paired markers common to most cells. N7, N9 and N10 are absent, and N11 is usually present in 4 copies.
- Tumorigenicity:
- yes
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3 – 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 90% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Viral contamination:
- HCV, HBV, HIV-1, HIV-2, EBV, CMV, HHV6, HPV not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms