English
SH-SY5Y
cell line / Homo sapiens / epithelial
- Lot number:
- SH-SY5Y-09-23
- Deposit date:
- 14.09.2023
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- ATCC CRL-2266
- Origin:
- Homo sapiens, subline of the neuroblastoma cell line SK- N-SH (ATCC HTB-11), obtained in 1970 from a metastatic bone tumor of a 4-year-old cancer patient
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO 11. D10S1248 14. D12S391 18, 22. D13S317 11. D16S539 8, 13. D18S51 13, 16. D19S433 13, 14. D1S1656 12. D21S11 31, 31.2. D22S1045 16, 17. D2S1338 17, 19. D2S441 11, 11.3. D3S1358 15, 16. D5S818 12. D6S1043 12, 18. D7S820 7, 10. D8S1179 15. DYS391 –. FGA 23.2, 24. GENDER X. SE33 19.2, 24.2. TH01 7, 10. TPOX 8, 11. vWA 14, 18. Yindel –
- Karyotype:
- Modal number = 47. the cells have a unique marker consisting of chromosome 1 with a complex insertion of an additional copy of the 1q segment into the long arm, resulting in trisomy 1q
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, gentamicin (20 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3 – 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 90% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms