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Acute monocytic leukemia THP-1
cell line / Homo sapiens / Acute monocytic leukemia
- Animal species:
- Human, Homo sapiens.
- Origin:
- blood
- Age:
- infant
- Tissue:
- Peripheral blood
- Morphology:
- monocyte
- Histological diagnosis:
- Acute monocytic leukemia
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- HLA antigens A2, A9, B5, DRw1, DRw2. lysozyme genes. HLA: (A2. A9. B5. DRw2), complement (C3), Fc.
- Tumorigenicity:
- yes, tumors in nude mice
- Contamination control:
- not detected
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- oncology, toxicology, immuno-oncology, model of normal differentiation (monocyte to macrophage).
- Culture method:
- in vitro adhesion, colonies in soft agar, in vivo (see Appendix 1).
- In vitro culture conditions:
- Medium – RPMI-1640 fetal bovine serum 10%. 2-mercaptoethanol 0.05 mM. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- by D.J. Giard
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- The cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin. Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA).
- Intended use:
- these samples are intended for laboratory research only.