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NCI-H23 [H23]
cell line / Homo sapiens / epithelial cells
- Lot number:
- NCI-H23-06-20
- Deposit date:
- 11.06.2020
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- CRL-5800 (ATCC)
- Origin:
- Homo sapiens, male, 51 years old, non-small cell lung cancer, adenocarcinoma
- Morphology:
- epithelial cells
- Species identity control:
- STR profiling CSF1PO: 10. D10S1248: 15. D12S391: 15, 17. D13S317: 12, 14. D16S539: 11. D18S51: 14. D19S433: 12, 14. D1S1656: 14. D21S11: 30. D22S1045: 16. D2S1338: 18, 23. D2S441: 11. D3S1358: 15. D5S818: 12, 13. D6S1043: 12. D7S820: 9, 10. D8S1179: 15. DYS391: – . FGA: 24. GENDER: X. SE33: 17. TH01: 6. TPOX: 8, 9. vWA: 16, 17. Yindel: –
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3 – 1:5
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 95% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms