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Melanoma B16
cell line / Mus musculus / melanoma
- Animal species:
- Mouse Mus musculus.
- Origin:
- melanoma
- Age:
- sexually mature animals.
- Tissue:
- skin
- Morphology:
- fibroblast-like
- Histological diagnosis:
- melanoma
- Carcinogenesis:
- spontaneous
- Average latent period (days):
- 20.
- Tumorigenicity (%):
- 95-100% in black mice C57BL/6J
- Optimal cell number:
- about 4×10⁵.
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Contamination control:
- bacteria, fungi and mycoplasma not detected.
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- oncology, screening, toxicology
- Culture method:
- in vitro partially adhesion/suspension, in vivo orthotopically or subcutaneously (see Appendix 1).
- In vitro culture conditions:
- medium – DMEM fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- Dr. Isaiah J. Fidler.
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- Transfection is possible, i.v. administration, they produce melanin (the culture medium turns dark brown or black).
- Intended use:
- these samples are intended for laboratory research only.