English
mel Lap
cell line / Homo sapiens / epithelial-like
- Lot number:
- LOT 2024-05-004
- Deposit date:
- 22.04.2024
- Number of cells per ampoule:
- 2,2×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Unique line
- Origin:
- Homo sapiens, male, 78 years old, skin melanoma (metastasis) Kosobokova E.N. et al. HUMAN METASTATIC MELANOMA CELL LINES PANEL FOR IN VITRO AND IN VIVO INVESTIGATIONS. Journal of Molecular Pathology. 2024. Vol. 5. No. 1. P. 11-27
- Morphology:
- epithelial-like
- Age (number of passages):
- passage 6
- Species identity control:
- STR profiling CSF1PO: 9, 11. D10S1248: 13, 15. D12S391:17, 19. D13S317: 8, 11. D16S539:12. D18S51: 14. D19S433: 12, 14. D1S1656: 12, 15. D21S11: 30.2, 31. D22S1045: 11, 14. D2S1338: 23, 25. D2S441: 11, 14. D3S1358: 15, 17. D5S818: 11. D6S1043: 19. D7S820: 10, 14. D8S1179: 12, 13. DYS391: 10. FGA: 19, 25. GENDER: XY. SE33: 30.2. TH01: 6, 9.3. TPOX: 11. vWA. 15, 19. Yindel: 2
- Karyotype:
- 74~88,XXXXYYYY,-Y,-Y,-4,del(6)(q21),add(7)(q36),+7,del(9)(p21),-10,-10,-11,- 11,der(14)t(14.?),-17,-17,- 21,-22,-22, +1~4mar.inc
- Tumorigenicity:
- tumorigenic in Balb/c nude mice
- Phenotypic markers (expressed antigens):
- Melan A, PD-L1, ERα, TUBB3
- Mutations:
- BRAF+, KIT-, NRAS-, KRAS-, CDKN2A-, PTEN-,TP53-
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 92% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology Genomic characterization