English
mel Hn(2022)
cell line / Homo sapiens / epithelial-like
- Lot number:
- melHn-02-22
- Deposit date:
- 03.02.2022
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- no, unique line
- Origin:
- Homo sapiens, female, skin melanoma, lymph node metastasis
- Morphology:
- epithelial-like
- Species identity control:
- STR profiling CSF1PO: 10, 11. D10S1248: 14. D12S391: 15, 20. D13S317: 9, 13. D16S539: 11, 12. D18S51: 17. D19S433: 12, 15. D1S1656: 15, 17. D21S11: 28, 31. D22S1045: 15, 16. D2S1338: 17, 21. D2S441: 11, 11.3. D3S1358: 14, 19. D5S818: 10, 12. D6S1043: 13. D7S820: 10. D8S1179: 13, 14. DYS391: –. FGA: 20. GENDER: X. SE33: 15, 19. TH01: 9.3. TPOX: 8. vWA: 16, 18. Yindel: –
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 98% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms Genomic characterization