English
HepG2 (2d) 3D
three-dimensional cell culture of tumor tissue / Homo sapiens / hepatocellular carcinoma from the collection of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", Moscow
- Species:
- Homo sapiens sapiens (human)
- Tumor tissue:
- hepatocellular carcinoma, from the collection of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", Moscow
- Passage number:
- immortalized line
- 3D cell culture production method:
- cultivation in dishes with an ultra-low adhesive coating
- Initial cell concentration:
- 100 thousand cells per mL of medium
- Culture medium:
- DMEM/F12, 10% fetal bovine serum, 1× antibiotic-antimycotic
- 3D culture duration:
- 2 days
- Spheroid diameter:
- 111±82 µm
- Spheroid shape:
- irregular, with a loose edge
- Spheroid fusion tendency:
- high
- Contamination control:
- bacteria, fungi, mycoplasma not detected
- Cryopreservation medium:
- 90% fetal bovine serum, 10% DMSO
- Karyology:
- hypotriploid line, modal chromosome number 55
- Application area:
- experimental oncology