English
Hep 3B
cell line / Homo sapiens / epithelial
- Lot number:
- Hep3B-12-23
- Deposit date:
- 25.12.2023
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Hep 3B2.1-7 [Hep 3B, Hep-3B, Hep3B], ATCC
- Origin:
- Homo sapiens, boy, 8 years old, hepatocellular carcinoma
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO: 8. D10S1248: 15. D12S391: 17. D13S317: 12, 14. D16S539: 10. D18S51: 20. D19S433: 12.2, 14. D1S1656: 16.2, 17.2. D21S11: 30, 31. D22S1045: 14. D2S1338: 21, 25. D2S441: 10, 12. D3S1358: 15. D5S818: 13. D6S1043: 12, 17. D7S820: 8, 10. D8S1179: 12. DYS391: –. FGA: 18. GENDER: X. SE33: 20, 23.2. TH01: 6, 7. TPOX: 9. vWA: 17. Yindel –
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640 or EMEM, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:4 – 1:6
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 85% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms