English
Human glioblastoma G01
cell line / Homo sapiens / brain
- Animal species:
- Human, Homo sapiens.
- Origin:
- Glioblastoma
- Tissue:
- brain
- Morphology:
- epithelial
- Contamination control:
- not detected
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- toxicology, oncology
- Culture method:
- in vitro, in vivo (see Appendix 1).
- In vitro culture conditions:
- Medium – DMEM fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- in 2022 Federal State Autonomous Institution N.N. Burdenko National Medical Research Center of Neurosurgery, Ministry of Health of Russia
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery"
- Patent, certificate:
- no
- Note:
- suitable for transfection, spheroids, oncology, toxicology
- Intended use:
- these samples are intended for laboratory research only.