English
G34O
three-dimensional cell culture of tumor tissue / Homo sapiens / squamous cell carcinoma of the floor of the mouth (biopsy, clinical material)
- Species:
- Homo sapiens sapiens (human)
- Tumor tissue:
- squamous cell carcinoma of the floor of the mouth (biopsy, clinical material)
- 2D cell culture isolation method:
- enzymatic dissociation
- Passage number:
- 1
- 3D cell culture production method:
- cultivation in dishes with an ultra-low adhesive coating
- Initial cell concentration:
- 100 thousand cells per mL of medium
- Culture medium:
- DMEM/F12, 10% fetal bovine serum, 1× antibiotic-antimycotic
- 3D culture duration:
- 10 days
- Spheroid diameter:
- 250±132 µm
- Spheroid shape:
- regular, with a distinct edge
- Spheroid fusion tendency:
- low
- Contamination control:
- bacteria, fungi, mycoplasma not detected
- Cryopreservation medium:
- 90% fetal bovine serum, 10% DMSO
- Karyology:
- 2n=46 (XX)
- Application area:
- experimental oncology