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Mouse kidney epithelium M 1
cell line / Mus musculus / kidney epithelium
- Animal species:
- mouse Mus musculus transgenic for the SV40 early region (tg(SV40E)Bri7).
- Age:
- sexually mature
- Tissue:
- kidney epithelium. collecting ducts, cortex
- Morphology:
- epithelial, adherent
- Histological diagnosis:
- kidney epithelium
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- antigens of the collecting ducts of the cortex
- Tumorigenicity:
- yes, tumors in nude mice
- Contamination control:
- not detected
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- toxicology, screening
- Culture method:
- in vitro adhesion, in vivo (see Appendix 1).
- In vitro culture conditions:
- Medium – EMEM fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- transfection is possible. The cells have characteristics of either intercalated cells or principal cells of the collecting ducts. 5 to 10% of cells show a dual phenotype of intercalated-principal cells. When grown on permeable supports, the cells develop a lumen-negative transepithelial potential difference.
- Intended use:
- these samples are intended for laboratory research only.