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Ependymoma EPNT-5(mus)
cell line / Mus musculus / ependymoma
- Animal species:
- Mouse Mus musculus.
- Origin:
- ependymoma 5 (5)
- Age:
- sexually mature animals.
- Tissue:
- brain.
- Histological diagnosis:
- ependymoma
- Carcinogenesis:
- 7,12-dimethyl-5,6-benzo[a]anthracene (intracerebral).
- Number of passages:
- 22.
- Average latent period (days):
- 35.
- Tumorigenicity (%):
- 80-100% in black mice C57BL
- Optimal cell number:
- about 4×10⁵.
- Contamination control:
- bacteria, fungi and mycoplasma not detected.
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Molecular profile:
- nicotinic and muscarinic acetylcholine receptors, diazepam receptors.
- Cloning efficiency:
- 60%
- Karyology:
- 2n= 40, range of variation in chromosome number 57-64, modal chromosome number 59-60, number of markers - 3-5 large metacentrics and 1 medium acrocentric with a secondary constriction (routine staining), number of polyploids 1.5%.
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Application area:
- neuro-oncology, cell biology.
- Culture method:
- in vitro monolayer, in vivo orthotopically or subcutaneously (see Appendix 1).
- In vitro culture conditions:
- medium – DMEM with glutamine. IDMI with glutamine. fetal bovine serum 10% passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 60%, 10% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- obtained in the Neuromorphology Laboratory of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery".
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery".
- Author:
- Research Institute of Human Morphology
- Patent, certificate:
- no
- Intended use:
- these samples are intended for laboratory research only.