English
DU 145
cell line / Homo sapiens / epithelial
- Lot number:
- DU145-12-23
- Deposit date:
- 25.12.2023
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- DU 145, ATCC
- Origin:
- Homo sapiens, male, 69 years old, prostate carcinoma
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO: 10, 11. D10S1248: 12, 13. D12S391: 18, 21, 22. D13S317: 12, 14. D16S539: 11, 13. D18S51: 12. D19S433: 13. D1S1656: 15, 16, 17.3. D21S11: 30, 33. D22S1045: 16. D2S1338: 16, 17. D2S441: 9, 14. D3S1358: 16. D5S818: 10, 13. D6S1043: 10, 11. D7S820: 7, 10, 11. D8S1179: 13, 14. DYS391: 10. FGA: 22. GENDER: XY. SE33: 19.2. TH01: 7. TPOX: 11. vWA: 17, 18. Yindel: 2
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640 or EMEM, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:4 – 1:6
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 87% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms