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CaCo2-THP1 1:2 (7d) 3D
three-dimensional cell culture of tumor tissue / Homo sapiens / culture 1 – intestinal adenocarcinoma, culture 2 – monocytes from the collection of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", Moscow
- Species:
- Homo sapiens sapiens (human)
- Tumor tissue:
- culture 1 – intestinal adenocarcinoma, culture 2 – monocytes, from the collection of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", Moscow
- Passage number:
- immortalized lines
- 3D cell culture production method:
- cultivation in dishes with an ultra-low adhesive coating
- Initial cell concentration:
- 100 thousand cells per mL of medium
- Cell ratio:
- CaCo2:THP1 1:2
- Culture medium:
- DMEM high glucose, 10% fetal bovine serum, 1× antibiotic-antimycotic
- 3D culture duration:
- 7 days
- Spheroid diameter:
- 250±87 µm
- Spheroid shape:
- irregular, with a distinct edge
- Spheroid fusion tendency:
- medium
- Contamination control:
- bacteria, fungi, mycoplasma not detected
- Cryopreservation medium:
- 90% fetal bovine serum, 10% DMSO
- Application area:
- experimental oncology