English
Multipotent blasts K-562
cell line / Homo sapiens / Chronic myeloid leukemia (multipotent hematopoietic malignant cells that spontaneously differentiate into recognizable precursors of the erythrocytic, granulocytic, and monocytic lineages).
- Animal species:
- Human, Homo sapiens.
- Origin:
- Chronic myeloid leukemia
- Age:
- elderly person
- Tissue:
- Bone marrow
- Morphology:
- lymphoblast, suspension
- Histological diagnosis:
- Chronic myeloid leukemia (multipotent hematopoietic malignant cells that spontaneously differentiate into recognizable precursors of the erythrocytic, granulocytic and monocytic lineages).
- Carcinogenesis:
- spontaneous from pleural effusion during blast crisis
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- CD7 (25%), isoenzymes AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, PGM3.Expression of bcr/abl
- Karyology:
- The stemline chromosome number is triploid, with the 2S component occurring in 4.2% of cases. Fifteen markers (M1 and M(15)) were present in almost all S-metaphases. Spontaneous nonspecific dicentric changes occurred, but rarely. Unstable markers were also rare. X was disomic and N9 was nullisomic. The Philadelphia chromosome occurs less often. was not detected in any of the 15 metaphases examined.
- Contamination control:
- not detected
- Viral infection:
- Epstein-Barr nuclear antigen not detected
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- oncology, 3D cell culture, Study of immune system pathology, Immunology
- Culture method:
- in vitro partially adhesion, in vivo (see Appendix 1).
- In vitro culture conditions:
- medium – IMEM fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- J. Ponten and associates from 1966 -1969 , Allen, 2016.
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- transfection is possible, highly sensitive target in vitro for natural killer analysis. studies confirmed that this is human erythroleukemia. Others indicate that (erythro, myelo, lympho oncoproliferation).
- Intended use:
- these samples are intended for laboratory research only.