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Adenocarcinoma HeLa
cell line / Homo sapiens / Adenocarcinoma
- Animal species:
- Human, Homo sapiens.
- Origin:
- cervical adenocarcinoma
- Age:
- sexually mature
- Tissue:
- cervix
- Morphology:
- epithelial
- Histological diagnosis:
- Adenocarcinoma
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase (JNK1) activity via a protein kinase C-independent pathway. keratin positive by immunoperoxidase staining, Isoenzymes G6PD,A
- Tumorigenicity:
- yes, tumors in nude mice
- Karyology:
- Model number = 82. range = 70 to 164. 98% of cells have a small telocentric chromosome. 100% aneuploidy in 1385 cells examined. Four typical HeLa marker chromosomes have been reported in the literature.
- HeLa marker chromosomes:
- one copy of M1, one copy of M2, four to five copies of M3 and two copies of M4, as shown in the G-banding figures. M1 is a rearranged long arm and centromere of chromosome 1 and the long arm of chromosome 3. M2 is a combination of the short arm of chromosome 3 and the long arm of chromosome 5. M3 is an isochromosome of the short arm of chromosome 5. M4 consists of the long arm of chromosome 11 and the arm of chromosome 19.
- Note:
- suitable for transfection, susceptible to Human adenovirus 3 Encephalomyocarditis virus Human poliovirus 1, 2 and 3. HeLa cells contain human papillomavirus 18 (HPV-18) sequences. P53 expression was low, and normal levels of pRB (retinoblastoma suppressor) were found.
- Contamination control:
- not detected
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- oncology, toxicology, can be used for screening Escherichia coli strains with invasive potential.
- Culture method:
- in vitro adhesion, colonies in soft agar, in vivo (see Appendix 1).
- In vitro culture conditions:
- Medium – EMEM fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- obtained in 1951
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Intended use:
- these samples are intended for laboratory research only.