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Breast adenocarcinoma4T1
cell line / Mus musculus / breast adenocarcinoma
- Animal species:
- mouse Mus musculus .
- Origin:
- breast adenocarcinoma, stage 4
- Age:
- sexually mature
- Tissue:
- mammary gland
- Morphology:
- epithelial, adherent
- Histological diagnosis:
- breast adenocarcinoma
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- resistant to 6-thioguanine
- Tumorigenicity:
- yes, tumors in BALB/c mice, metastasizes
- Contamination control:
- not detected
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- 3D cell culture, Immuno-oncology, Tumors induced by 4T1 can be used as a postoperative model as well as a non-surgical model, since the 4T1-induced tumor metastasizes spontaneously - the best model of human stage 4 metastatic breast tumor.
- Culture method:
- in vitro partially adhesion, in vivo (see Appendix 1).
- In vitro culture conditions:
- medium – RPMI-1640 fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum. (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- transfection is possible. can metastasize to the lungs, liver, lymph nodes and brain, while the primary tumor grows in situ. The primary tumor does not have to be removed to induce metastatic growth. Resistant to 6- thioguanine, micrometastatic cells (as few as 1) can be detected in many distant organs with greater accuracy than in most tumor models. There is no need to count nodes or weigh target organs.
- Intended use:
- these samples are intended for laboratory research only.